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An oxygen-sensitive sensor: ruthenium-tris(4,7-diphenyl-1,10-phenanthroline) dichloride (Ru(DPP 3Cl2), the phosphorescence of which is quenched by molecular oxygen, was synthesised according to a method modified by us and then applied.
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Lipid isolation from culture media was carried out using a chloroform/methanol (2 : 1, v/v) solution according to Folch method modified by [ 16].
Heparinized (20 IU.ml-1) blood was drawn from healthy laboratory personnel and separated according to a method originally described by Boyum [ 48], slightly modified.
The β-glucosidase activity was determined according to a method previously described by Berghem and Pettersson [ 20], which was slightly modified.
The activity of ASA was assayed according to Roy's method modified by Błeszyński and Działoszyński [ 19].
YP hydrolysis was carried out according to a modified method by Zambrowicz et al. (2013).
Liver tissue (100 mg) was prepared for hydroxyproline (Hyp) determination according to a modified method by Jamall et al. [ 18].
Mannitol dehydrogenase (MDH) activity was determined according to a modified method [ 36].
Total lipid extraction was carried out according to a modified method.
Hippocampal dissociated neurons were prepared according to a partly modified method of that previously described [30].
GLAP was synthesized according to a slightly modified method of Usui et al. [ 28].
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