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The dried samples were stored under nitrogen at -20°C until processed for TLC and GC-FID analysis according to a method established by Mylnefield Lipid Analysis.
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Circulating endothelial cells were measured in 1 ml whole blood by four-color FCM according to a method we previously established (Jacques et al, 2008).
The LDH activity was determined according to the method established by Tarmy et al. [ 34].
The EC was determined according to the method established by the International Honey Commission [ 4].
HF19 cells were plated in 24 cm flasks (Nunc, Denmark) at dilutions adjusted to yield approximately 100 viable colonies according to the method established by Puck and Marcus (1956).
To produce a population-based respondent sample, random-sampling software was developed by a statistician (X-T C), according to the EPI method established by WHO [11].
We obtained a representative sample of the Chinese adult population using random-sampling software according to the EPI method established by WHO [21].
The AUCs were computed using the non parametric trapezoidal method and their 95% confidence limits were computed according to method established by DeLong [ 28].
HUVEC were separated from HBMSC by applying magnetic beads coupled with an antibody against CD31, a specific protein of ECs according to the methods established by Guillotin et al [23].
Lytic units (LU20/10 cells) of the assays were calculated according to the methods established by Bryant et al. [ 31].
These congeners were analyzed according to the routine methods established by national authorities [ 37].
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