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Serum MDA levels were analyzed based on the reaction with thiobarbituric acid by high-performance liquid chromatography (HPLC) according to a method developed by Katepe [29].
For in vitro experiments, mid-veins were pulled out from leaf laminae according to a method developed by Brown et al. (2010), and both mid-veins and residual leaf laminae were immediately frozen in liquid nitrogen, and stored at −80 °C.
PAL was systematically estimated for each subject according to a method developed by Johansson et al. [ 26].
Each birth defect is also classified as major or minor according to a method developed by the Centers for Disease Control and Prevention [ 17, 18].
Phase-dependent local dynamic stability was defined according to a method developed by Ihlen et al. [ 16] and based on two equations: (1) λ = ln d i t s t e p t, (2) λ = 1 t n ln d i t n s t e p d i 0 s t e p, where 〈 d i (t)〉 is the reaction curve of the initial perturbation 〈 d i (0)〉 and the outer brackets 〈⋯〉 are the mean across all steps in the walking bout.
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Ocular hypertension was induced according to a method developed in our laboratory.
The ability to reduce ferric ions was measured according to a modified method developed by Benzie and Strain (1996).
Socioeconomic status was estimated according to residential postcodes using a method developed by the Australian Bureau of Statistics: the Index of Relative Socio-Economic Disadvantage [ 27].
Total carotenoid content was estimated from the yellow pigment content, extracted and measured according to a micro-method developed by Belaggi et al. Antioxidant activity in both the free and bound fractions was estimated using two different methods.
The behavioral responses were observed and quantified by a second experimenter according to the method developed by Vos et al. [33].
BC was measured by a reflectance technique according to the method developed by Reid et al. (1998).
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