Chromatography and quantitation have typically been accomplished using high-performance liquid chromatography mass spectrometry (HPLC-MS) in negative ion mode, with quantitation of the parent ion (LC-MS) or transition ions (HPLC-MS/MS).
Production of such functional antibodies requires the correct glycosylation of the CH2 domain, which until recently could only be accomplished using higher eukaryotic cells (Nguyen et al. 2003) but not by microbial production.
Blocking was accomplished using a high block buffer containing 10% BSA, 10% goat serum, 0.1% Triton X-100 in 0.15 M phosphate buffer.
Extraction of DNA from 134 plasma samples was accomplished using the High Pure PCR Template Preparation kit (Roche, Lewes, UK).
This is accomplished using a high-sensitive sensing function in the route construction.
Conversion of total RNA to single-strand cDNA was accomplished using the High-Capacity cDNA Archive Kit (P/N 4322171; Applied Biosystems, Foster City, CA).
Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum.
Dual emission ratio imaging at 485 nm and 535 nm for Epac1-camps and dual excitation ratio imaging at 400 nm and 490 nm for roGFP2 were accomplished using a computer-controlled high speed filter wheel (Lambda 10-2 optical filter changer with rotation every 60 80 ms, Sutter Instrument Co., Novato, CA).
After isolation, subsequent fractionations have been accomplished using SPE or a high-pressure liquid chromatography (HPLC) system commonly fitted with a C18 reverse-phase column.
Acquisition of the temperature distribution on the heater surface was accomplished using an infrared [IR] high-speed camera, SC 6000 from FLIR Systems, Inc. (N. Billerica, MA, USA).
Immunolabeling of multiple components or live cell dynamic recording can be readily accomplished using fluorescence microscopy, while retrieval of high resolution structural data requires advanced electron microscopy.
