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The acclimation time for enrichment culture lasted for 40 days.
LT50 and LT90 of all life stages increased with increasing acclimation time to 42 °C in general.
The procedure of settling, decanting, addition of new effluent and nutrients was repeated at 24-h interval until the desired acclimation time (approximately 1 month) had been achieved.
We conducted a series of experiments to identify and quantify the influence of parasite infection and food source, food quality, body size and acclimation time prior to testing on the feeding rate of individual Gammarus pulex.
There is possibility of argument that the high rate of oxygen consumption in the initial 4 h of experiment under various parameters could be because of the artefacts of experimental procedures such as insufficient acclimation time.
We conducted a series of experiments to identify and quantify the influence of parasite infection and food source, food quality, body size and acclimation time on the feeding rate of Gammarus pulex.
Similar(45)
Our review identified 165 studies describing behavioural responses in fish exposed to various stressors, and revealed that the majority of publications documenting fish behavioural responses report extremely brief acclimation times and observational durations, which helps explain inconsistencies identified across studies.
Based on transcript response profiles, only members of groups I, III, IV and VII remained differentially expressed compared to control at the end of the acclimation time-period.
While it is unclear if the differences in HMGB1 regulation in T. bernacchii and A. limneaus are representative of the adaptive differences between the species, or potentially related to the difference in acclimation times used within the two studies, these data further supports the case for HMGB1 as an important regulator of thermal stress response in fish.
All three biological replicates of each sample (acclimation and time point) were measured in duplicate 25 µl reactions.
At the end of this period, control non-acclimated plants were harvested (NA0) or maintained under the same light and temperature conditions for 5 (NA5), 7 (NA7), 21 (Nand) and 45 d (NA45) to provide adequate controls for the different cold acclimation (CA) time points.
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