These accession experiments were independently conducted, with overlap of five accessions.
In contrast to the constrained major energy metabolism pathways, the aliphatic glucosinolate biosynthetic pathway showed significantly elevated pathway level transcript CV in both accession experiments suggesting that the transcripts in this pathway may be under selection for increased diversity.
The ratio SW/BM significantly differed among accessions (P = 1×10−5), but not between experiments, the interaction accession x experiment not being significant either (P≥0.100).
Comparison of virus accumulation in the two experiments showed a significant interaction between accession and experiment, suggesting that virus accumulation in each genotype depends on the developmental stage at the time of inoculation.
In addition, a significant accession x experiment interaction (P = 1×10−5) was detected, suggesting that the reduction of plant growth due to CMV infection depended on the time of infection for each accession.
In a second approach, to characterize the phenotype of the semi-dwarf accessions an experiment was conducted with 12 natural accessions (Table 1) together with the ga5 mutant and its parental wild type, L er.
SRA data listed in the table consist of experiment accession, study accession, title, submitter, technology, library source and library selection.
Microarray data are deposited at ArrayExpress, a public repository, under the following experiment accession number (ArrayExpress accession: E-MEXP-1973).
Details of microarray experiments 1 and 2 have been submitted to ArrayExpress and assigned accession numbers E-MTAB-1484 E-MTAB-1484 E-MTAB-1484AB-1478 (Experiment 2).
Two independent Q-RT-PCR experiments were conducted, testing three plants per accession in each experiment.
Access to data concerning this study may be found under GEO experiment accession number GSE15812 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi token=pjihtumyoyogmhy&acc=GSE15812.nih.gov/geo/query/acc.cgi token=pjihtumyoyogmhy&acc=GSE15812
