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We chose to exclude SNPs that were less that 90% homogeneous within either accession 1) to provide a reduced set SNPs that could discriminate between the two subspecies and 2) to allow for some degree of sequencing error at the SNP position when there was low sequence coverage.
For the Musa ornamental diploid species represented by M. basjoo (accession 1; Table 1) and the hybrid 'Royal' (224), derived from a cross between two species of the section Rhodochlamys (M. ornata x M. velutina) [ 59], a slightly larger fragment than the 350 bp from M. balbisiana and the 530 bp from the M. acuminata fragment were observed.
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First we examined publicly available EST sequence data for RASSF1A and RBSP3 (for RASSF1A Accession No. NM_007182; RBSP3AccessionioNo.o. AJ575644, and for RBSP3B, Accession No. AJ575645).
The microarray data are accessible from the Gene Expression Omnibus (Accession GSE22834).
We named these two NPR1-like sequences, GmNPR1-1 (AccessioNo.o. FJ418595) and GmNPR1-2 (AccessioNo.o. FJ418597).
The four Indian cultivars––A.D.T.4 (accession 415), A.D.T.14 (accession 417), M.T.U.9 (accession 420), and P.T.B.8 (accession 421)––showed weakness in hybrids with P.T.B.7, indicating that these four cultivars also carry Hwa1-1Hwa1-1
A total of 2088 and 12,141 unigenes were preferentially expressed in prostrate-growing wild accession C792 and erect-growing wild accession C793, respectively.
RNA-Seq raw data for the six libraries (NCBI Accession: PRJNA413795), representing reads from the control samples T1-T3 (NCBI Accessions: SRX3277329, SRX3277330 and SRX3277331) and the 10% ethanol-stressed samples TE1-TE3 (NCBI Accessions: SRX3277326, SRX3277327 and SRX3277328) are available online.
The data has been deposited at DDBJ/EMBL/Genbank under the accession LNGJ00000000.
The raw data from these studies are deposited in GEO, accession GSE57181 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE57181).nih.gov/geo/query/acc.cgi acc=GSE57181
Based on results of three-way cross combinations, Oka (1957) reported that P.T.B.10 (accession 414) and P.T.B.7 (accession 419) carry Hwa1-1 and Hwa2-1, respectively, whereas Pei-ku (accession 108), Padi ase banda (accession 647), and Kissin (accession 521) carry neither Hwa1-1 nor Hwa2-1.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com