The physical basis for this resistance is difficult to interpret as this minor proportion of PrPC was readily accessible to degradation by subsequent exposure to PK (Figure 1C).
To check whether the protein fusion is accessible to factor Xa cleavage and subsequent release of its GFP moiety, GTXG-VLPs (288 µg protein/ml) were treated with Triton X-100, thereafter incubated with factor Xa and subsequently subjected to SDS-PAGE and western analysis.
An effective pretreatment should open up the LCB cell wall matrix, hydrolyse the hemicelluloses, reduce cellulose crystallinity and ultimately make the cellulose and hemicellulose more accessible to the enzymes in the subsequent hydrolysis process that converts the carbohydrate polymers into fermentable sugars [ 2, 3, 10].
The relevant articles were triaged in an online reference RefWorks site accessible to all members, for more manageable ongoing discussions and subsequent referencing.
Written language was a big breakthrough in 3,000 B.C., enabling bookkeeping, publishing and to-do lists; the subsequent invention of phonetic alphabets made it accessible to the masses, not just the hacker elite.
Subsequent treatment with saponin renders intact phagosomes accessible to antibodies (Fig. S1C) and allows detection of intra-phagosomal bacteria.
An official student-faculty committee that investigated the campus disturbance, however, did fault his administration, saying it had failed to make itself accessible to students and faculty members; their alienation, the panel said, contributed to the subsequent student strike.
Sakurai's subsequent philosophy for Brawl was for the game to be more readily accessible to new players.
Because in the case of soluble laminin and agrin the clustering process is arrested at the ovoid cluster stage, the subsequent differentiation of high density AChR membrane regions has not heretofore been accessible to in vitro studies of this type.
It was found that the amount of electrolyte accessible to extraction remains relatively constant during the first 25 cycles, but then continuously decreases within the subsequent 40 investigated cycles.
The subsequent paradigm shift from interfaces to the bare surfaces of TMOs made the confined electron system accessible to surface-sensitive spectroscopic techniques and this new era is the focus of the present article.
