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The difference between the decay kinetics of the different nanoparticles (ANOVA, p ≤ 0.05) could be explained by the presence of rutin nanocrystals adsorbed on the surface of the nanodroplets of nanoemulsions (as observed by TEM Fig. 1), making rutin more readily accessible to react with the ·OH free radical.
Control experiments in which the coupling reaction was carried out with DM-PaDDAH (i.e., lacking an additional Cys residue) yielded no reaction with the MDCC coupling reagent, indicating that none of the other cysteines in the protein are sufficiently solvent accessible to react with the fluorophore.
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The lack of candidate PLA2s among 4-BPB's putative targets may also be because, based on its chemical structure, 4-BPB is predicted to react with any accessible cysteine or histidine.
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