Both membrane leaflets of the plasma membrane and those of intracellular membranes, as well as the variety of fracture planes through lipid droplets, are accessible to labeling.
Their two-dimensional extension made the FG repeat films accessible to label-free and quantitative analysis by a toolbox of surface-sensitive biophysical methods.
The N-terminal amino group and two lysine residues present in both Aβ1-40 and scrambled Aβ40-1 peptides may be accessible to labelling with Cy5.5 dye.
A third cysteine residue in the construct (Cys) is buried in the core of the structure and is not readily accessible to labelling (see below).
On the other hand, some previous studies that included AA identities of the contacts have used arbitrary cut-off values on the associated solvent accessible surface areas to label the contacts as exposed or not [ 15].
Forty-eight hours after transfection, HEK293 cells were washed with prewarmed uptake buffer (37 °C, pH 7.4) and then incubated with either uptake buffer alone or uptake buffer containing 10 mM MTSES or 10 mM MTSES and 1 mM estrone-3-sulfate or 10 mM MTSES and 0.75 mM estradiol-17β-glucuronide for 10 min at 37 °C to label the accessible cysteines.
Set up small receptacles in an accessible area in your kitchen, and make sure to label the receptacles accordingly: paper/cardboard, plastic, glass and aluminum/steel.
There are a variety of routes by which you can get into analytical science, prompting Greenacre to label it as "possibly the most accessible of the sciences".
We use 'a' to label inaccessible residues and 'A' to label accessible ones.
Given that the release of processed APP from a synaptic vesicle by exocytosis (either in the form of sAPPα or Aβ) would require the N-terminal end of the protein to be orientated towards the lumen of the vesicle, it is the C-terminal end of the protein that should be most accessible to antibody labeling on the intact vesicle.
The unprecedented radical site identified in this work as Tyr71 is close to the surface, but not necessarily accessible to spin-labels or cross-linking reactions.
