Interestingly, CrSGD was targeted to the nucleus (Additional file 3i-l) with a specific aggregation pattern when its C-terminus extremity was accessible in the fusion protein (Additional file 3m-p) suggesting that an autonomous aggregation could occur.
Thus, the insertional-fusion technique increases accessible positions for gene fusion on RNase, resulting in construction of a potent cytotoxic RNase.
It is small, highly translated, and its tertiary structure reveals that both its amino and carboxyl termini are accessible for potential fusions to other molecules.
We made gourmet fusion food accessible to all.
With stacked cisternae, only the rims are accessible for budding and fusion, but as cisternae unstack, more membrane would become available so that the flux of material through the stack could increase.
To check whether the protein fusion is accessible to factor Xa cleavage and subsequent release of its GFP moiety, GTXG-VLPs (288 µg protein/ml) were treated with Triton X-100, thereafter incubated with factor Xa and subsequently subjected to SDS-PAGE and western analysis.
Inhibition of the late stages of cell fusion by lowering the concentration of accessible PtdIns(4,5) P2 in the plasma membrane is consistent with the hypothesis that CGPs drive the expansion of fusion pores.
This fragment holds a His6 tag sequence for C-terminal fusion to an inserted gene and is accessible for cloning via a BspEI site.
The finding that changes in the concentration and/or activity of diverse proteins involved in shaping intracellular membranes and in the membrane concentration of accessible PtdIns(4,5) P2, an important regulator of membrane shaping, affect late fusion stages substantiates the hypothesis that CGPs drive fusion pore expansion in cell-to-cell fusion.
Cellfish.com's Fusion Art channel provided users with live access to the workshop, which was accessible from a PC as well as mobile handsets.
