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Charged, hydrophilic, sulfhydryl reagents have been used to probe systematically the accessibility of substituted cysteines in putative transmembrane segments of a number of proteins.
Here, we selected OAADPr as the substrate because of the accessibility of isotopically substituted reactants and because of the link to Sirtuin pathways.
The accessibility of blood makes it an attractive substitute for brain tissues, but there is so far little direct evidence for its suitability for this purpose.
Conformational changes in GPCR (FRET-based measurements) upon addition of a hypotonic stimulus and consecutive receptor activation have been described for BK2R in bovine aortic endothelial cells [ 59], for PTH1R (FRET-based) in pre-osteoblastic cells [ 60], and for AT1R (determined by means of substituted cysteine accessibility mapping) in HEK293 cells [ 61].
For the VDR-DBD, however, the T147 is substituted by a hydrophobic phenylalanine residue, that in order to optimize its packing and limit accessibility of surrounding waters, recruits the aliphatic portion of the R160 chain from VDR.
Accessibility of controls?
Impact hinges on the accessibility of research.
She helped fundamentally change the price and accessibility of fashion.
I like the humanity and accessibility of it.
They also looked at style, plot and accessibility of language.
Adequate diagnosis and accessibility of treatment is another problem.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com