A total of 108 microsatellite markers covering 21 chromosomes, with an average distance of 23 cM between markers, were selected according to accessibility of the markers in the first genetic consensus map [ 18] and heterozygosity of the F1 parents.
Despite the accessibility of established molecular markers in major crops such as soybean, it is often difficult to find a sufficiently dense set of polymorphic markers for a specific biparental cross.
A total of 961 primer-pairs were designed targeting 767 different unigenes carrying microsatellite repeats in the expressed component of the sugarcane genome, which would extend the accessibility of such microsatellite markers to researchers for many genetic studies in sugarcane.
This comparison of breakpoint density to an experimental marker of genome accessibility provides additional support to the hypothesis that a major determinant of BPR localisation is open chromatin.
Second, an area's proportion of workforce in agriculture was used as the proxy indicator of access to pesticides; other variables may be better markers of pesticide accessibility, e.g. pesticide sales, for which small-area data were unavailable.
Most of the older species-specific molecular maps were constructed with RFLP markers, but in recent times there has been increased utilization of PCR-based markers because of accessibility and higher throughput.
The iceberg phenomenon of CD (i.e., an assumed large number of undiagnosed cases) remains an important concern in children and adolescents despite increased disease prevalence and awareness, highly sensitive and specific CD serologic markers, and improved accessibility of upper GI endoscopy and biopsy.
Now that a methodology for generating transgenic chickens has become available [ 15], it is likely that the availability of useful markers and the accessibility of chick embryos for live imaging will significantly increase the use of avian models and will boost research in this area.
DNaseI hypersensitivity serves as a measure of chromatin accessibility and is therefore a reliable marker for sites of histone disruption and associated regulatory activity.
Perhaps as a result of the increased accessibility to NGS technologies for STR marker development, there has also been an increased level of activity in the development of associated software for identifying candidate markers.
We aimed to develop alternative synthetic actin markers with improved synthetic accessibility and reduced toxicity; these being the main limits of the few existing compounds.
