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Lyso-PC was not accepted as substrate.
Butylfumarate is not accepted as substrate by either MAL.
We tested whether lactose is accepted as substrate and whether using lactose as a substrate could lead to GOS formation.
Collectively, these results suggest that the flanking residue is not critical for activity and that the minimal length of the peptide to be accepted as substrate is four residues.
It may be possible that the acyl-ACP produced by AAS can also be accepted as substrate in addition to acyl-CoA by jojoba FAR in engineered Synechocystis strains.
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These results also indicate that well-designed non-natural amino acids carrying relatively large side chains can be accepted as substrates of the translation system.
The fact that all of these heterocyclic systems were accepted as substrates by the enzyme and gave normal Baeyer-Villiger products broadens the synthetic utility of the engineered E. coli strain and emphasizes the chemoselectivity achievable with enzymatic oxidation catalysts.
Norcamphor (13) and -cis-bicyclo [3.2.0] hept-2-en-6-one (14) were better accepted as substrates than camphor in general, and furthermore (R,R -bicyclo [2.2.1] heptane-2,5-dion (15), which is stR,R -bicycloimilar to the natural substrate 2,5-diketocamphane (3), is also converted.
Bulky alkenes such as (E -stilbene, or compounds with E -stilbenethdrawing grorp direcompoundsected to the terminal alkene, such as (E)-4-phenylbut-3-en-2-one, (E)-1-(3-chloroprop-1-enyl) benzene, and (E)-1-(2-nitrovinyl)benzene with not accepted as substrates of the SMO (Lin et al. 2010, 2011b).
As shown in Table 3, tetrapeptide L-Ala-γ-D-Glu-meso-A2pm-D-Ala and pentapeptide L-Ala-γ-D-Glu-meso-A2pm-D-Ala-D-Ala were also accepted as substrates, but less efficiently.
Methylamine and ethylamine are also accepted as substrates, showing ∼12% and <1% conversion after 2 h of incubation, respectively.
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