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Each female was given either one Sarcophaga host and one Protocalliphora host in a host preference (i.e. choice) experiment or two Sarcophaga in a host acceptance experiment.
The number of silverfish individuals differs in some colonies between the multivariate analysis of all behavioural categories and the analysis of the aggression index as some individuals did not complete the standardised number of 50 ant contacts for the social acceptance experiment as some silverfish were seized by ant workers (Table 2).
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However, when the laboratory strains of both species were presented with two Sarcophaga hosts in acceptance experiments, both strains were highly accepting of Sarcophaga (84%, N=89 for N. vitripennis and 78%, N=79 for N. giraulti).
For each silverfish individual host aggression was quantified first via social acceptance experiments (standardised contact study), and then its CHCs were extracted and subjected to chemical analysis.
Preliminary tests of bkbwg/bkbwg homozygous females showed a complete failure to successfully parasitize Sarcophaga and Protocalliphora hosts in both the Sarcophaga and Protocalliphora host choice experiments and the two Sarcophaga acceptance experiments (data not shown).
In the two Sarcophaga acceptance experiments, bkbwg/+v heterozygous females did not show a significantly reduced contact rate relative to N. giraulti (χ1=0.45, p=0.42) but they did show a significantly reduced stinging rate (χ1=4.1, p<0.05).
We therefore focused our host choice and acceptance experiments on bkbwg/+v heterozygous females, and as an additional control, we tested the preference of mutant N. vitripennis strain peach (the genetic background of the bkbwg introgression line).
This suggests that the older, more senior student participants showed less of an emotional response to the task and showed greater preservation of positive affect, possibly due to greater acceptance of experiment participation in general, and not simply due to the design of the task itself.
But don't call acceptance an experiment.
As part of the progression toward regulatory acceptance, toxicogenomics experiments should ideally be conducted in accordance with GLP.
An anticoincidence system surrounds the PAMELA silicon tracker to reject particles not clearly entering the acceptance of the experiment.
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