Exact(11)
DNA methylation was measured at baseline, week 7, and week 14 using a [3H]methyl acceptance assay and a novel liquid chromatography tandem mass spectrometry assay of the DNA bases methylcytosine and cytosine.
Global DNA methylation was assessed by methyl acceptance assay.
Global DNA methylation status was assessed by methyl acceptance assay (Chen et al. 2004).
We determined genomic DNA methylation by the methyl acceptance assay using the method of Balaghi and Wagner (1993).
Genomic DNA methylation was determined in colonic mucosa using an in vitro methyl acceptance assay (Balaghi and Wagner, 1993).
We determined global DNA methylation by the methyl acceptance assay, as described previously (Benbrahim-Tallaa et al. 2005), at 0, 20, and 40 weeks of cadmium exposure.
Similar(49)
Thirteen groups were found to have some kind of criteria implemented for assay acceptance.
First guidelines for validation and training beyond this publication as well as for assay acceptance are in preparation.
Furthermore, acceptance criteria for assay performance were only used by a limited number of laboratories, and each criterion was unique for the laboratory that used it.
31 Acceptance of the assay was based on the consistency in staining patterns and a coefficient of variation (CV) among sample scores that does not exceed 20%, although larger CVs observed at lower H-scores may be acceptable.
Using the 10-fold serial dilutions of a pool of these 96 RNA transcripts (pool 11 in Table 1), limit of quantification, linearity, precision and accuracy for each of RNA transcript will be determined, and the acceptance criteria of assay performance for each RNA transcript will be established.
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