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The simplest rule demonstrating the highest sensitivity with acceptable level of specificity was selected as the final tool.
RAPIDR has been validated using >700 samples and delivers an acceptable level of specificity and sensitivity for T21, T18, T13 and monosomy X.
The average recovery of the added recombinant VEGF165 ranged between 85 115%, indicating an acceptable level of specificity of the assay.
Among all the proposed models, the one that showed the highest level of sensitivity and an acceptable level of specificity and that fit the ease-of-use criterion was selected in order to develop the screening tool.
We performed selection of the minimal number of probes which achieved the highest possible sensitivity (Histologic Grade HG3 patients correctly identified) and an acceptable level of specificity (HG1 patients correctly classified), in order to have accurate grade assignments.
While the choice of the final predictive model could have been made from several criteria, we decided that the rule demonstrating the best sensitivity and an acceptable level of specificity would be the most appropriate because such a tool could identify patients at risk with fewer false negatives.
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However, the performance of these criteria is such that it is difficult to combine acceptable levels of specificity and sensitivity.
The results of the ROC analyses showed that, with acceptable levels of specificity, the sensitivity of nerve conduction tests would not be very high.
The idea is that the computer may be able to improve a radiologist's, or radiographer's, sensitivity while relying on his or her professional judgement to maintain acceptable levels of specificity.
There is adequate evidence to speculate that targeting HIF-1α could improve the long-term prognosis of these patients In order to increase the overall sensitivity of the immunoassay, maintaining acceptable levels of specificity, a panel of antibodies should be used.
Since we noted that HIF-1α assessment can be antibody-dependent, a prudent future approach would be to use a panel of antibodies in order to increase the overall sensitivity of the immunoassay, at the same time maintaining acceptable levels of specificity as we did in our study.
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