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Our results show that partial reduction of HIP/RPL29 levels accelerates differentiation of C3H/10T½ into cartilage-like cells.
At an early stage of myoblast differentiation, increased expression of Barx2 upregulates the expression of several myoblast differentiation markers including α-smooth muscle actin (SMA) and accelerates differentiation [30], [31], while in myotubes, ectopic expression of Barx2 appears to suppress the expression of differentiation-associated genes (Fig. 1, 3).
Furthermore, TLR activation accelerates differentiation of committed osteoclasts, and promotes mature osteoclast survival [ 39- 41].
In utero knockdown of Imp1 cell-autonomously reduces cell proliferation and accelerates differentiation of neural stem/progenitor cells.
Moreover, knockdown of endogenous c-Myc strongly accelerates differentiation, an effect that is significantly reversed by introduction of the miRNA mixture.
Over-expression of miR-1 [ 55] or miR-206 [ 86] in mouse myoblasts accelerates differentiation into myotubes whereas over-expression of miR-133 proliferationiferation [ 55].
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If dead stele cells were occasionally observed in controls, their number clearly increased after IR (Fig. 1-B and C), therefore indicating an accelerated differentiation of protoxylem and/or their differential sensitivity to IR compared to ground tissue.
These results demonstrate that the MC3T3 cells used for microarray analysis differentiated appropriately and that the HDIs accelerated differentiation as expected from our previous studies [ 18].
Conversely, knockdown of NO66 in differentiating osteoblasts derived from mesenchymal stem cells causes accelerated differentiation and mineralization of osteoblasts [ 40].
Our work has established defined ex vivo culture conditions for enhanced hMSC multicelluar aggregation and accelerated differentiation with the potential to deliver either undifferentiated or pre-differentiated cells to the site of injury to aid repair.
This accelerated differentiation may be due to a non-antigen-specific effect in RA that differentiates all peripheral T cells irrespective of their specificity, or it may actually reflect an antigen-specific expansion of T cells potentially driven by autoantigen.
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