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AC clearance, C, (ml/min) is the rate at which AC is leaving the blood (i.e. the rate of whole body AC uptake) divided by the concentration of AC in the blood.
The main objective of this study was to compare peripheral AC uptake in participants with normal and high fasting insulin concentrations and the ability of AC to reduce FFA in the two groups.
Since, during the IV AC infusion, when serum AC has reached a steady-state concentration, the rate of whole-body AC uptake, U, is equal to the rate of infusion, therefore: [3] C = 1000 × I / [ AC ss ] where I is the AC infusion rate (mmol/min) and [ACss] is the steady-state serum AC concentration (μmol/L).
Densitometric measurements of AC uptake into inner ear otoliths (optical density of AC/μm2) revealed that the kinetotic individuals had incorporated significantly more AC/calcium than the normally behaving fish.
Therefore, this study is aimed at comparing AC uptake in NI and HI participants.
In conclusion, this study shows that AC uptake is similar in NI and HI participants.
Similar(36)
Interestingly, approximately 55% of sorted muscle EC were negative for ac-LDL uptake while muscle EC positive for ac-LDL comprised only 45% of all ac-LDL+/CD45− cells (figure 2C).
Acetylated low-density lipoprotein (ac-LDL) uptake was performed by adding 10 µg/ml ac-LDL labeled with rhodamine (Biomedical Technologies Inc ,Stoughton, MA).
Acetylated low density lipoprotein labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindo-carbocyanine perchlorate (DiI-Ac-LDL) (Biomedical Technologies) was used to characterize ac-LDL uptake by hECFCs and HMVECs at varying oxygen tensions.
In vertebrates, co-expression of endothelial and myogenic markers in combination with acetylated low-density lipoporotein (Ac-LDL) conjugated with the fluorochrome probe Dil (Dil-Ac-LDL) uptake is associated with a population of myoendothelial cells located in the muscular interstitial spaces and involved in muscle regeneration [6], [7].
For DiI-ac-LDL uptake assay, hESC-ECs were incubated with 10 µg/ml of DiI-Ac-LDL (Molecular Probes, Eugene, OR) at 37°C for 6 hours.
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