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Despite abundant transcripts, the level of intracellular PfEMP1 was low suggesting post-transcriptional mechanisms to partake in protein expression.
The existing data suggest that for relatively abundant transcripts the existence and direction (but not the magnitude) of expression changes can be reliably detected.
To prevent over-representation of the most abundant transcripts, the resulting double-stranded cDNAs were normalized using the Kamchatka crab duplex-specific nuclease method (Trimmer cDNA normalization kit, Evrogen) [32].
To reduce the presence of overly abundant transcripts, the TRIMMER kit (Evrogen, Moscow, Russia) was utilized.
In the context of abundant transcripts, the post blood meal digestion midgut infected with L. infantum chagasi is relatively quiescent.
Despite the large difference in number of differentially abundant transcripts, the functional themes that distinguish exponential and stationary phases in Lange et al and this study are similar.
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For this reason, the amplification was biased towards the high abundant transcripts and the counts of the low abundant transcripts showed a high variation.
Coding sequences in the genome represent a variety of transcription levels, with several abundant transcripts occupying the majority of the mRNA pool of the cell.
In the first strategy, the normalization process very effectively increased low abundant transcripts, since the bulk of the novel unigenes described (4673) derived from this library.
He believed the university underrated his chosen speciality, but he contributed powerfully to its recognition when, using the abundant transcripts of the Nuremburg trials, he wrote his Hitler biography.
Similarly, the more abundant transcripts in the remaining transcriptome received most of the remaining reads, and so on (Fig. 3).
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