Sentence examples for abundant spot from inspiring English sources

The relatively abundant spot indicated by an arrowhead in Figure 1a and 1b was excised manually from a colloidal Coomassie Blue-stained preparative gel and digested with trypsin.

These spots have an expected ratio of 1, which is also the expected ratio of the more abundant spot type TRG that will have more weight in defining the value of the optimal threshold.

Some low abundant spots were pooled from different gels to increase the signal in mass spectrometric analysis.

For identification of proteins from 2D-PAGEs, the most abundant spots were excised and digested with trypsin as described above.

In adult worms, 26 of the 40 most abundant spots were identified, and corresponded to 22 different proteins.

The majority of the low abundant spots in UT FN and HW FN were detected in the second or third replicates (Additional file 8).

The present investigation of cytosolic proteomes of NCFM grown on cellobiose, a potential synbiotic combination, and glucose identified a total of 136 differentially abundant spots in 2D-DIGE.

In addition, considering that gel imaging analysis represents a compromise between the detection of minor spots and the limitation of saturated abundant spots, we used two different staining procedures, Coomassie and silver nitrate.

On the level of proteins the number of detected variants exceeded the number of detected transcripts in case of Obp3 and several other Obp proteins were detected in low abundant spots.

Analyzing the differently abundant protein spots by spot intensity quantification using the PDQuest software, we found a total of 21 over-expressed proteins in patients than in control subjects (considering an expression difference >1.5-fold).

Several protein spots have been identified as cytoskeletal proteins, including actin as most abundant protein spot (E48) on the 2D gel and tubulin.