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We therefore systematically analyzed the restriction map of rice CDSs and selected seven abundant restriction sites that are not present in the Ubi promoter; at least two of the sites are absent in 99.55% of the rice CDSs (Table 1).
Finally, a third possibility for increasing tag density would be to choose an endonuclease that recognizes more abundant restriction sites in the wheat genome.
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Abundant restriction-modification systems were determined.
As many marine bacteria harbor multidrug resistance genes and abundant restriction-modification systems which make genetic manipulation rather difficult (Wang et al. 2015), this selection approach eliminates the need for targeted genetic manipulation or random mutagenesis.
This is the most abundant motif in Type II restriction enzymes characterized thus far and is often found in DNA repair and recombination-related enzymes.
In this study, the assembly produced 251,816 contigs, an amount that was too high, indicating that EcoRI restriction sites were abundant on the genome of G. hirsutum.
The average interval of recognition sites of EcoRI and BamHI was 3.8 and 7.9 kbp, respectively, suggesting that in the silkworm genome EcoRI restriction sites were more abundant than BamHI restriction sites.
Mining of the variant data revealed a total of 2395 polymorphic restriction sites, the most abundant being those revealed by TaqI (438), AluI (401), RsaI (381), DpnII (321), HinfI (281) and HaeIII (147).
The Flyfisher's Guide to Connecticut states that the Beaver Brook is a class 1 Wild Trout Management Area and is described as having abundant wild trout with no seasonal restrictions, but is catch and release only.
Nevertheless, although abundant literature has evidenced that dietary sodium restriction decreases BP levels in patients with essential hypertension, no large and prospective study has been conducted to date on this issue in patients with CKD.
Such strong bands represent highly amplified restriction fragments and correspond to abundant repetitive sequences in the representation; a feature which is highly detrimental to DArT performance [ 32].
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