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We assayed success of depletion (~ 98%) of major proteins by visualizing less abundant ones on silver-stained gels (data not shown).
An interesting finding was the inverse relationship seen between some isomers or among closely related compounds in which the more abundant isomers or family member were positively correlated with phenylpropanoid transcript abundance, but the less abundant ones were negatively correlated.
On the downstream mass spectrometric level, the excess of highly tryptic peptides generated from these abundant proteins bias the identification towards these proteins on the expense of lower abundant ones.
We did not detect changes in these low-abundance proteins, because our analytical procedure favors detection of more abundant ones.
Nevertheless, since the majority of the low abundance viruses appear in the same groups as the highly abundant ones in our phylogenetic analyses, these viruses are most likely associated with arthropods.
The benefit of using Simpson's index over alternative diversity indices is that it has low sensitivity to species at low abundance (in this case genomes), and gives most weight to the most abundant ones.
Most viral research focuses on strains amenable to laboratorial manipulation, rather than the most relevant or abundant ones [8].
But for everyone, there were satisfactions, and abundant ones.
This exhibition proves that grasping at straws, even abundant ones, can become tedious.
The NHS has to prepare for a stretch of modest years after so many abundant ones.
Those organisms with an ability to synthesize scarce monomers, say A, from more abundant ones, say B, would have persisted.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com