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The abundant matched b- and y- ions, accurate precursor ion mass, and expected mass difference from the SILAC pair observed in the spectrum correlated well with the low expectation value or p-value reported by algorithms.
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The most abundant matches are those to repetitive elements already known to exist in Glycine max.
These miRBase miRNAs were flagged as possible orthologs and each was assigned a pig miRNA sequence identical to the most abundant matching read sequence.
As expected, a large fraction of the most abundant tags matched with widely expressed mitochondrial genes or ribosomal proteins such as ribosomal proteins P1 and S26.
In HLA-A (only spanning reads, n = 203), the most abundant haplotype matched the transmitted haplotypes of the parents NA12891 and NA12892, which both transmitted an identical haplotype.
In the 23 DAP library, 6 of the 30 most abundant tags matched in sense as well as in antisense orientation to the Brassica EST dataset, oriented based on the A. thaliana proteome.
For two of the miRNAs (miR-29a and miR-29b) the most abundant sequence matched with the mature sequence listed in miRNA databases such as miRBase (http://www.mirbase.org), with a residual presence of variant sequences with 5′ trimming, 3′ trimming, substitutions and 3′ additions.
Most of these molecules, as predicted, are low-abundant siRNAs matched to various classes of repeats or genomic regions (Fig. 1) [36].
In category I, the miRNA-guided cleavage fragment was the most abundant tag matching the transcript; In category II, the miRNA-guided cleavage fragment was not the most abundant fragment; however, it still formed a clear peak in the T-plot.
Compared to the planar model of revolute joints only with radial clearance, journal motion inside the bearing is more abundant and matches the reality better in this model.
Compared to the planar model of revolute joints only considering radial clearance, the journal motion inside the bearing is more abundant and matches the reality better in the EEM.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com