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Enzymatic removal of pectic homogalacturonan from equivalent sections resulted in the abundant detection of distinct patterns of the LM15 xyloglucan epitope across these organs and a diversity of occurrences in relation to the cell wall microstructure of a range of cell types.
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The most abundant detection method, however, is coimmunoprecipitation which accounts for nearly half of all evidence.
Semi-quantitative analysis was performed after laser scanning densitometry in comparison to different amounts of standard human recombinant IL-1Ra, and scored as follows: -, no detection; +/- very faint and/or inconsistent detection; + consistently positive; ++ abundant detection; +++ very high levels.
A more detailed description of the putative secreted proteins follows, starting with proteins encoded by the most abundant transcripts: Detection of a protein in the proteome that matches the predicted peptide sequence of the transcript is denoted under Protein detected by "Y" Thus far, SP15-like proteins have only been reported in sand flies and their function remains unknown.
Amongst the abundant miRNAs identified, detection of miR-124a-1 was especially important as it is often cloned at very high frequencies in the brain [ 18, 25, 41].
This will minimize abundant proteins from masking the detection of less abundant ones, and will permit full qualitative and quantitative analyses.
These abundant proteins may hinder the detection of low-abundant proteins that can be of specific interest in the search for biomarkers of disease [ 8].
However, since kinases are of relatively low abundance, interference from highly abundant proteins hampers the detection of kinases, rendering the studies of global kinome by MS very challenging.
Abundant literature treats the detection of different Control Chart Patterns (CCPs).
However, compared to the liver and muscle, where glycogen is abundant, the sensitivity for detection of brain glycogen by 13C-MRS inherentlytlowlow.
In mammalian cells, host protein synthesis is drastically reduced at 6 h after infection, allowing detection of abundant late protein bands as shown in Fig. 8C.
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