However, they can be approximated using abundant expression datasets, for example utilizing normalized variation among microarray assays between replicates of populations [ 42, 43].
Inversely, mRNA reads that were assigned to the Clostridium and Haemophilus genera were more abundant in dataset B (9% and 2.5%, respectively) compared to dataset A (2% and 1%, respectively).
The (A/T n mono-nucleotide repeat motif was the most abundant in both datasets.
Also abundant in our datasets were loci encoding two transmitter domains and multiple receiver domains.
Although we cannot extract evidence of the strength of prezygotic isolation between toad species from the current dataset, abundant evidence from anuran vocalization studies suggests that prezygotic isolation could be quite important, and may have driven speciation in this group in a manner similar to that of birds [16], [76] [78], [80].
As a result, the actin transcript levels were around 32-fold more abundant than the dataset's average.
Alternatively, maSigPro can miss genes with less pronounced changes, which can be recovered by ASCA-gene if their profile is abundant within the dataset.
The most abundant in this dataset were metabolic enzymes and nutrient transport genes, consistent with this tissue's physiological function in digestion (Table S3 in Additional file 2).
However, RT domains belonging to the Ty3/gypsy, Copia, and the recently identified red/aquatic species (RAS) lineage [ 32] are the most abundant in the dataset analyzed.
While the neutral fraction of the distribution is not known, a robust estimator for the variance should be only minimally affected by selected SNPs unless they are very abundant in the dataset.
