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In order to detect the least abundant candidate (Myt1; 1.3×10−4% of the screened cDNA library) by cloning and sequencing, it would have been necessary to analyze at least 1.0×106 clones.
Based on that, the most abundant candidate miRNAs were further examined by northern blot to test for expression.
An example of the nucleotide conservation of the 80 most abundant candidate sRNAs is shown in Figure 5.
We used northern blot analysis to confirm the expression and determine the sizes of these sRNAs, with strand-specific probes for the 25 most abundant candidate sRNAs with predicted sigma 70 promoters (ECS001-025; Additional File 4).
A comparison with conclusions obtained from NMR solution studies of zeaxanthin and lutein geometrical isomers is in good agreement with the most abundant candidate structures proposed in this study.
The LRZ was analyzed using an Arizona microarray, which provided abundant candidate genes with diverse functions that are postulated to play important roles in adaptation to low-phosphate conditions.
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Sediments like clay are well known for providing such barriers, and at depth, phyllosilicates of the clay, mica, and serpentine groups are abundant candidates for providing similar barriers.
Cloud services consumers face a critical challenge in selecting trustworthy services from abundant candidates, and facilitating these choices has become a critical issue in the uncertain cloud industry.
We assessed these most abundant candidates in F. cylindrus by Northern blot but only two probes (Fc-3 and Fc-4) gave a positive result.
The seven most abundant candidates all mapped to loci that were annotated as tRNA or rRNA except Fc-2 (Additional file 2), although some also mapped to other loci that were not annotated.
The precursor sequence of the most abundant miRNA candidate in adult ovaries, bge-candidate 1, was characterized by this method (Figure 5A B) and only a few copies of its miRNA* could be found in Ov-A library (see Text S2).
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