The phrase "abundance target" is correct and usable in written English.
It can be used in contexts related to goals or objectives that focus on achieving a surplus or plentiful amount of something, often in business or economic discussions.
Example: "Our company has set an abundance target for the next fiscal year to ensure we maximize our resources and output."
Alternatives: "surplus goal" or "plentiful objective".
Our results might be specific to the protein kinase family we have investigated but suggest the need for improved methods for the more rapid recovery of low abundance target proteins and their interacting proteins.
Moreover, there are some [37] that question whether 18S represents the best reference control when normalizing a low abundance target gene that crosses the threshold at 24 cycles relative to the very abundant 18S with a CT value of 9 cycles (Table 1), corresponding to a 32,000 fold-difference in gene expression.
We conclude that the CRAC technique can identify even low abundance targets of the surveillance machinery.
Quantitative MS approaches will also serve to confidently identify lower abundance targets, thus expanding the known repertoire of TgCDPK1 targets.
However, even relatively low abundance targets are well represented in the data set, showing that the CLASH approach is not limited to abundant mRNAs.
Nevertheless, the uniform sampling of transcripts by deep sequencing considerably limits the precision achievable by RNA-Seq for low abundance targets.
Based on our findings, targets at a low abundance may only be detectable by particles at relatively low shear stress, while higher abundance targets may be detected across a broader range of shear stress conditions.
Peptide adducts with electrophiles have been characterized and mapped on target proteins with tandem mass spectrometry scans and SALSA software (Chapter 13); finding low abundance targets and focusing instrument time on peptides of highest interest are big challenges.
The use of more sensitive proteomics techniques with DARTS should greatly enhance its capability to identify lower abundance targets, although which exact methods will prove most useful is to be determined.
DOI: http://dx.doi.org/10.7554/eLife.03640.017 To obtain more direct evidence for the readthrough-dependent low abundance targeting of human LDHB to peroxisomes, we analyzed untransfected wild-type cells by immunofluorescence with anti-LDHB and anti-PEX14 antibodies.
Assays with very low abundance targets Ct > 37.4 (4% of cortex samples, 15% of hipocampal samples) were judged not abundant enough for precise quantification and were assigned an expression value of zero.
