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The important consideration is that the core components remain somewhat constant and the relative abundance range of more abundant species remains within range and does not fluctuate so much as to give different interpretations of results.
The average coefficient of variation for abundance for this group of 49 proteins was 56%, which is excellent given that the abundance range of this protein population spans 3 orders of magnitude.
Interestingly, the abundance values of proteins in this core proteome span the full abundance range of the entire NB4 proteome.
To test how reproducible this calculation may work we analyzed a mixture of 48 recombinant proteins distributed over an abundance range of 6 orders of magnitude.
Therefore, we ranked the quantified proteins into four abundance classes over a dynamic abundance range of approximately five orders of magnitude (Fig. 1).
The measured abundance range of the proteins subjected to ELISA is indicative of the depth of proteomic analysis in this study, which was achieved through extensive fractionation of intact proteins and reliance on high-resolution MS, spanning seven logs of protein abundance.
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However, because a significant fraction of cell surface proteins are transmembrane and have a relatively low abundance and solubility [17], differential extraction is required to reduce the abundance range and the complexity of the samples to acquire good quality results.
When considering the entire gene set, 61% and 37% of genes exhibited the highest mRNA abundance in fraction C (18 to 42% abundance range with a mean value of 25 ± 4%) or in fraction H (18 to 34% abundance range with a mean value of 23 ± 3%), respectively.
Furthermore, we defined an abundance-window of 33% around a target protein as a localized background for calculating the statistical significance, by which we can evaluate the significant bias of a target protein-abundance compared to the abundance-distribution range of its neighbored proteins rather than to the abundance-distribution range of all identified proteins.
The results, collated year by year, offer a window on trends in the abundance and range of species ranging from the snow bunting to the sandhill crane.
Hermit crabs play an important role in the Northern Adriatic Sea due to their abundance, wide range of symbionts, and function in structuring the benthic community.
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