The phrase "abundance of template" is not correct in standard written English.
It may be intended to describe a large quantity of templates, but the phrasing is awkward and unclear.
Example: "The project requires an abundance of templates to ensure all scenarios are covered."
Alternatives: "large number of templates" or "plenty of templates".
Local abundance of repair template may direct the repair toward HDR instead of NHEJ.
Telomere length was measured as abundance of telomeric template vs. a single gene by quantitative real-time PCR.
The lower the abundance of any template, the smaller the probability is that its true abundance will be maintained in the amplified product.
Telomere length was measured as abundance of telomeric template (T) vs. a single gene copy (S) by quantitative real-time PCR as described previously [ 68].
In a series of initial calibration experiments we demonstrate how the barcode tiling approach can quantify the abundance of individual template molecules from a pool of 305 shRNA expression constructs.
The relative abundance of the template was calculated from the Ct values and shown as percentage of the maximal expression during the experiment.> Identical amount of cells were collected from two independent cultures for each strain at different times, as indicated in the Results.
Telomere length was measured as abundance of telomeric template versus a single copy gene (36B4) using the following primers: TelA (5′-CGG TTT GTT TGG GTT TGG GTT TGG GTT TGG GTT TGG GTT-3′); TelB (5′-GGC TTG CCT TAC CCT TAC CCT TAC CCT TAC CCT TAC CCT-3′); 36B4F (5′-CAG CAA GTG GGA AGG TGT AAT CC 3′) and 36B4R (5′-CCC ATT CTA TCA TCA ACG GGT ACA A-3′).
This information was used for further PCR bias correction and to estimate the abundance of sequenceable templates in each sample.
This physical linkage of templates with hypothesized amplification biases was included to allow a direct assessment of the magnitude of such biases, given the known shared abundance of paired templates.
Because annealing and amplification efficiency cannot be perfectly matched among primer pairs, read counts can reflect PCR bias in addition to bona fide differences in the abundance of TCR templates.
