The phrase "abundance in the initial" is not correct and does not convey a clear meaning in written English.
It may be intended to describe a plentiful or ample amount at the beginning of something, but the phrasing is awkward and unclear.
Example: "There was an abundance in the initial stages of the project, which allowed us to move forward quickly."
Alternatives: "plenty at the outset" or "ample at the beginning".
An optimal upscaling of mRNA that introduced minimal systematic biases, would maintain the relative transcript abundance present in the initial mRNA sample.
However, the most important issue for any amplification protocol to be used in combination with quantitative analysis of gene expression, is that the relative transcript abundance present in the initial mRNA sample is maintained throughout the procedure.
For most of the samples, the apparent copy number abundances observed in the initial analysis of individual donor samples (Fig. 5) were also observed in the repeated analysis, in that ALUJ and TERT genomic copies exceeded those of RPHH1, which in turn were higher than those measured for ERV3 (Fig. 7; see also the electronic supplementary material).
As expected the MFs in these five clusters decreased in abundance between the D0 and M1 time points, and either continued to show decreased abundance at later time points or slightly increased in abundance after the initial D0 to M1 decrease.
When comparing the relative abundance of amoA in the initial community of the successful cultivation to the unsuccessful community (Additional file 1: Figure S6), there is 1.69 log2 fold greater representation of amoA in the unsuccessful cultivation.
The results showed certain members of the bacterial electrode community increased in relative abundance from the initial inoculum.
Relative quantification was calculated using the comparative threshold (CT) cycle, which is inversely related to the abundance of mRNA transcripts in the initial sample.
The present work demonstrates that the difference in the ability to detect the three genes above depends on the sample purity and the initial abundance in the pure B-cell sample.
By the eighth week there were no differences in abundance according to the initial dilution level (Supplementary Information; Fig. S1).
In this case, it is a big advantage to present the data in a form in which the abundance of both the parent and daughter isotopes are given with respect to the abundance of the initial background daughter.
