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In order to further confirm the specificity of positive IFA results, three plasma samples were studied in an absorption assay.
Cross-agglutination absorption assay identified the unknown serovar from serogroup Australis as a new serovar closely related to Lora (11 ).
To evaluate the specificity of the FilGAP antibody in IHC analysis, absorption assay was performed, as described previously 15.
In conclusion, we have developed a rapid, continuous three-enzyme coupled UV absorption assay for the characterization of enzymes that use SAM to catalyze methyl transfer reactions.
Interestingly, unbound C-EBV collected from the AK31 absorption assay could infect both AK31 and CNE-2 cells with similar infection rates, like the unbound C-EBV from CNE-2 cell supernatants.
To examine the specificity of the anti-FilGAP antibody on formalin-fixed and paraffin-embedded sections, absorption assay was performed using normal human kidney tissues which have the highest expression in podocytes 10, 19.
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In absorption assays, antibodies against H pylori were removed to determine the role of molecular mimicry.
Plasma absorption assays with whole washed bacteria were performed essentially as described [23], with hirudin as anti-coagulant.
Protein samples incubated for various lengths of time at pH 1.7 in the presence of 360 mM NaCl were also analyzed using the ThT fluorescence and the CR optical absorption assays.
Antiserum pre-absorption assay was performed prior to membrane incubation.
Furthermore, a peptide pre-absorption assay was also performed for the Western blot analysis revealing that the SNAI1 and SNAI2 bands were no longer detectible using pre-absorbed antisera (Figure S2D).
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