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The antiradical activity was determined using the following formula: Percent % inhibition of DPPH activity = A 0 - A 1 / A 0 × 100 % Where A0 is the absorbance value of the blank sample or control reaction, and A1 is the absorbance value of the test sample.
Samples were first kept in a dark place at room temperature and their absorbance was read at 518 nm after 30 min. The antiradical activity was determined using the formula below: Percent % inhibition of DPPH activity = A 0 – A 1 / A 0 ] × 100%% Where A0 is the absorbance value of the blank sample or control reaction, and A1 is the absorbance value of the test sample.
Similar(58)
The radical scavenging activity of the tested samples, expressed as percentage inhibition of DPPH, was calculated according to the following formula: (1) IC = [ A 0 − A t A 0 ] × 100, where A t is the absorbance value of the tested sample and A0 is the absorbance value of blank sample at a particular time.
Each of 1%, (w/v) SDS, 1%, (w/v) cetrimide and 1%, (w/v) tween were tested, hopefully they may increase the absorbance value of the reaction product.
The cut-off value was calculated as the mean absorbance value of the negative controls plus three standard deviations, as obtained with a single NASBA-ELISA test.
The absorbance value of the protein sample was corrected by the absorbance of a blank and the absorbance of the interference.
The Adye is the absorbance value of the dye at its peak absorbance.
Nitrite concentrations were determined by comparing the absorbance values for the test samples to a standard curve generated by serial dilution of a stock solution of sodium nitrite.
Absorbance values of the treated cells were compared with the absorbance values of untreated cells.
The average absorbance values of the samples and controls, each in 5 repeats, were calculated by subtraction of the background absorbance from the measured absorbance.
The absorbance values of phage samples were extrapolated with the standard graph made from the absorbance values of the known phage standards from the kit.
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