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A dependence of absorbance on electrolytic charge during the potential step was derived.
The different values of the minimum absorbance on each substrate were most likely due to the different reflection spectrum of the metal substrate.
The dependence of the absorbance on pH shows that as the pH increases the protonation of the amino groups decreases.
The polarization-dependent spectra were pretreated by a procedure to eliminate from the total absorbance the component shaped solely by the specific dependence of the ATR absorbance on the effective penetration depth.
In this paper, the dependence of the change in absorbance on the charge consumed during electrochemical preparation of Prussian blue (PB) film electrode has been studied by in situ spectroelectrochemical technique.
In order to clarify the potential of the method of autofluorescence for determining the collagen content in meat batter, an experimental design was used where the emission originating from collagen was unrelated to the effect of myoglobin absorbance on the emission spectra.
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The dependences of absorbances on time at the wavelengths corresponding to polaronic and exitonic transitions of the polymers were deduced.
Our method was tested based on this artificial blood absorbance, not on the absorbance of real blood shown in Fig. 1.
The amount of Pi produced in the kinase reaction was calculated from the absorbance based on the calibration curve.
All assays were performed in 96 well formats or from cell lysates, and luminescence, fluorescence or absorbance measured on a Synergy HT BioTek plate reader (BioTek Instruments).
The sample was subjected to absorbance test on a spectrophotometer at a wavelength of 690 nm.
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