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Exact(31)
% Inhibition = 100 - Absorbance of test compound - Absorbance of blank / Absorbance of control - Absorbance of blank × 100.
A sample: the absorbance of test extract.
Aλ 1 and Aλ 2 denoted the absorbance of test wells.
The DPPH free radical scavenging activity was calculated using the following formula: Scavenging activity % = Absorbance of control - Absorbance of test sample Absorbance of control × 100.
Aλ1 and Aλ2 represent absorbance of test wells at 540 nm and 630 nm, respectively.
The net optical density (OD) values were determined by subtracting the absorbance of test serum against negative control antigen from the absorbance of test serum against the DENV-1 antigen.
Similar(29)
Absorbance of tested samples at critical wavelengths (290, 308, 330 and 350 nm) [ 30] were selected for comparison.
The % inhibition was calculated as: % Inhibition = [ AControl − ASample)/ AControl] × 100 Where AControl is the absorbance of the negative control and ASample the absorbance of tested samples or standard.
Positive results for CFT were defined as titers of ≥1 : 4 and IgG ELISA absorbance ≥0.25; IgM was positive at a positivity index (absorbance of tested sample/absorbance of cut-off control) of >1.1.
The antioxidant activity (E) of the tested samples was calculated by determining the decrease in absorbance at different concentrations by using the following equation: Where At and Ac are the respective absorbance of tested sample and ABTS+, [ 19].
The absorbances of test and control (Ac) were read at 412 nm against distilled water.
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CEO of Professional Science Editing for Scientists @ prosciediting.com