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It was observed that the absorbance of the model apple solution and levels of the suspended particles (turbidity) affected UV dose.
Considering the Mie linear approximation of reflectance as noted in Eq. (4), the similarity with PCA results is found as stated by Eq. (8): ln (R = p c 1 - p c 2 ln - p c 2 K (8 Being K the exponent of hemoglobin absorbance on the model and pc n would be the PCA scores, as defined in Eq. (4).
Table 3 Analysis of variance of calculated model for atorvastatin absorbance Source Absorbance Mean squares F p-value Model 0.19 100.37 0.0001 Residual 9.417 × 10-003 - - R 2 0.9945.
A quadratic model for absorbance at 1041 nm was established and validated.
A linear model for absorbance was obtained using the least squares method.
It was proposed to describe degradation effect using absorbance change, and prediction model of pesticide residues degradation was obtained according to the relationship between degradation time and absorbance.
The integral for the absorbance spectrum of each model shows that the absorption gains reach up to the highest point in period of 0.5 μm, which is 46.44%.
Active AP-1 was quantified by reading absorbance at 450 nm (model 680, Bio-Rad).
Active NF-κB was quantified by reading absorbance at 450 nm (model 680, Bio-Rad).
The HPLC system consisted of a Shimdzu LC-9A pump and a Model 3200 absorbance detector (Shimadzu Scientific Instruments, Princeton, NJ, USA) set at 316 nm.
Nuclear proteins were extracted from the cells after a 1.5-h treatment, and 4 μg of protein was used to quantify the activity of NF-κB and AP-1 by reading absorbance at 450 nm (model 680, Bio-Rad).
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