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Traditional methods of absorbance ratios or HABA filtrations are of limited utility due to the large UV absorbance cross-section of QDs.
Under state II conditions, PSI associates with a mobile pool of the LHCII antennae, which increases its absorbance cross-section (Kargul et al., 2005; de Bianchi et al., 2010).
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Under other wavelength ranges σi would differ because the absorbance cross section for photoinactivation is dependent upon wavelength [29], [41].
The volumetric absorbance cross section (m/l), however, remained more or less constant throughout the entire experiment.
In addition to a change in absorbance cross section, the absorbance spectrum, and thus the pigment class composition, changed drastically.
The absorbance cross section of the biomass was measured and used as a proxy for the pigmentation.
In addition, red chlorophylls increase the absorbance cross section of the entire complex, especially in shaded environments.
Periodically, samples were taken aseptically and analyzed for dry weight concentration, biomass composition, residual dissolved nitrate, Fv/Fm ratio, absorbance spectrum, and absorbance cross section.
An up to eightfold decrease in the biomass specific absorbance cross section (m/g DW) was observed at the end of the cultivation compared to the point before nitrogen depletion in both the wt and the slm1.
This suggests that the decrease in biomass specific absorbance cross section is mainly a result of dilution of pigments over newly formed biomass and is likely caused to a lesser extent by net degradation of pigments.
The decrease in absorbance cross section between the slm1 and wt was similar, but the slm1 showed a higher ratio of absorbance at 483 nm/680 nm as the nitrogen starvation progressed.
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