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Empty wells were also incubated with neutral red dye to allow for background absorbance correction.
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The chapter also discusses the background-absorbance correction and refractive-index effects.
Correction (absorbance of untreated extract) and control (absorbance of reagents without extract) were also measured.
Correction (absorbance of the untreated extract) and control (absorbance of the FRAP reagent) were also measured.
The quantification was carried out in a standard less mode using atomic number, absorbance, and fluorescence correction factors with the dedicated software (ESPRIT version 1.9; Bruker) for the elements Cl, Ca, O, Na, P and Si.
The image calibration and correction to absorbance was done automatically in the Evince software package as described in Williams et al. [ 26].
The concentration of MDA was calculated from the absorbance at 532 nm (correction was made by subtracting absorbance at 600 nm for turbidity) by using extinction coefficient of 155mM-1 cm-1.
Also, the spectra were subtracted from their own baselines (using the spectral subtraction option provided in the instrument software) and converted to absorbance mode then baseline corrected by choosing the 'automatic baseline correction' option in the software.
Here additionally, we introduced a correction for the absorbance of light by EGCG to obtain reliable KD values.
In addition, the average transmittance for PBS buffer alone is higher than the base transmittance through the oil and was therefore used as a blank for correction of the absorbance baseline.
The absorbance was measured with a wavelength correction (A450 nm) with a microplate reader (Bio-Rad).
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