We propose that SPD-2 could act as a general protein-recruitment factor, which is only absolutely essential for the recruitment of centriole-duplication factors and PCM proteins to the sperm centriole(s) after fertilization.
On one hand it was suggested that as mutant forms of Dsh that are not recruited to membranes by Fz receptors can still mediate canonical signaling, Dsh recruitment was absolutely required only for planar polarity activity (Axelrod et al. 1998; Boutros et al. 1998; Rothbächer et al. 2000; Simons et al. 2009).
The head of marketing at one of the former 1994 Group universities recently summarised his position during an informal chat with me: "We are definitely looking to take advantage of the opportunity for additional undergraduate recruitment, but absolutely need to plan carefully and make sure we attract the students who are going to benefit most from the experience here".
A further point from our experiments is that we find that BIG1 is not absolutely required for the recruitment of GGAs to membranes.
Although phosphorylation of L-plastin does not appear to be absolutely required for its recruitment to actin polymerization sites, it might, by increasing the pool of high affinity L-plastin, contribute to the fine-tuning of actin turn-over.
Both motifs are absolutely required for Rad1 recruitment to 3′ flaps in SSA, and thus SSA repair (Li et al., 2008, 2013).
Arf1 is absolutely required for the recruitment of COPI (coatamer protein I), AP-1 (adaptor protein-1) and GGA (Golgi-localizing γ-adaptin ear homology domain Arf-binding proteins) coat proteins to the Golgi.
We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm.
In contrast, GCP-WD is absolutely required for γ-tubulin recruitment and seems to function more proximal to the γTuRC in the recruitment pathway, most likely downstream of Cep215/pericentrin.
"The importance of companies mapping out their sub-contracting recruitment chain is absolutely critical and contractors have to accept that if workers are on your site then they are your employees, even if they are hired by someone else," said Wells.
"The fact we're absolutely floundering with ideas of recruitment - because ideas can't come from me and Matt [Williams, club secretary] - we need players in and there is no sign of that on the horizon, that is one of our biggest problems, so that weighed heavily".
