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Seed germination of several of these GA-deficient mutants absolutely depends on the addition of GA to the medium during imbibition [ 17].
This again contrasts with Apc deficient intestinal epithelium which absolutely requires c-Myc to functionally manifest the phenotypes of Apc loss and tumour formation (Sansom et al, 2007).
Xeroderma pigmentosum group A protein (XPA) is a key subunit of the nucleotide excision repair pathway in mammalian cells that recognizes the increased deformability of damaged sites,[ 8] and is absolutely required for NER.[ 9] We used an XPA-deficient cell line (XP2OS [ 10] to examine the role of NER in the biocompatibility of click-linked DNA in human cells.
The presence of the central pair microtubules is absolutely required for motile cilia function within the brain: mice deficient in the central apparatus protein SPAG6 [ 5] lack the central pair microtubules and develop severe hydrocephalus.
No detectable infection was obtained with either virus in NPC1-deficient cells, indicating that filovirus entry into these cells is absolutely dependent on the E. helvum NPC1 ortholog.
A recent study using RIP1-deficient cells, however, challenged the common notion that RIP1 is absolutely required for TNF-induced NF- κB activation, suggesting that other ubiquitinated proteins within complex I can recruit and activate the TAB-TAK1 and the IKK α IKK β NEMO complexes.
However, Mbd3-deficient ES cells have been derived suggesting that NuRD contributes to but is not absolutely essential for epiblast establishment.
Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels.
"Yin deficient.
Is he mentally deficient?
"Absolutely, absolutely, absolutely, absolutely".
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