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"It is certainly an issue that has to be tackled, but for us the focus is the absolute viability of the plant first," he said.
As a general quality test the absolute viability was determined which is defined as the ratio of viable cell number after freezing to the total cell number before freezing.
To achieve a standardisation of freezing protocols we propose to control all process parameters including the nucleation temperature and the use of the absolute viability as a general method for quality comparison of different cellular specimens after freeze/thawing.
However, the absolute viability of poxviruses in well-preserved samples has not been determined.
If absolute viability is required, the loaded cells of interest can always be tested for their viability by following their division after recoding the fluorescence data.
The viability signals obtained at each time point are related to the initial (t = 0 hrs) value and the resulting temporal dynamics of the signal for each treatment condition can be evaluated as follows: a decrease below the initial value can be interpreted as a direct cytotoxic effect as the absolute viability signal decreases.
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In addition, our absolute sperm viability estimates are consistent with male sperm investment in singly mated females [14].
When using this method, the percentages of live and dead sperm may be affected by the dilution of sperm in artificial buffer, such that the obtained values represent relative, and not absolute, sperm viability and likely indicate the robustness of sperm when exposed to the buffer [5], [16].
We are fast approaching the absolute limit of viability.
P-values calculated for absolute change in viability.
That really is right at the absolute border of viability".
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CEO of Professional Science Editing for Scientists @ prosciediting.com