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Our mutational analysis suggests that at least GTP binding by Gpn2 and Gpn3 is an absolute requirement of cell viability; thus all three GPN proteins require GTP.
I insisted upon an absolute requirement of adhering to ABM and the necessity of instituting a nuclear weapons test ban...
The well-organized priesthood of the religion of Isis, for example, represented a serene sacerdotalism; sexual abstinence was an absolute requirement of those who celebrated her holy mysteries.
In short, Lurianism proclaims the absolute requirement of an intense mystical life with an unceasing struggle against the powers of evil.
ELISA assays confirmed the absolute requirement of the sulfate group for protein recognition and the potential application of this synthetic oligosaccharide as HNK-1 surrogate.
We note the challenges and difficulties in achieving the absolute requirement of quantitatively assessing the degradation of protein molecules in a drug delivery system.
This is the theme of many of these tales: the attempt to preserve a self when self-sacrifice -- on someone's part -- is an absolute requirement of the social order.
One feature of Kv7 channels is the absolute requirement of PIP2 binding to be functional and mounting evidence reveals that sensitivity to PIP2 is modulated by CaM binding30,31.
Integrity is the one absolute requirement of managers.
One characteristic feature of the AcrB trimer is the absolute requirement of functional cooperation between the three protomers (Eicher et al. 2014).
Moreover, proportionality is not an absolute requirement of justice – in some contexts (though probably not parking), the opportunity costs of achieving proportionality might be too great, taking resources that were more urgently required elsewhere to redress or reduce injustice.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com