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Likewise, absolute quantification analysis of cPMLΔ5&6 demonstrated that approximately 25% of total PML in virally infected cells are cPMLΔ5&6 (Fig. 2C, right panel).
For expression measurements, we used the absolute quantification analysis from the LightCycler Software 4.0 package (Roche), and calculated expression levels relative to wild type values.
For expression measurements, we used the absolute quantification analysis from the LightCycler Software 4.0 package (Roche), and calculated expression levels relative to values of a reference sample.
The evaluations were performed on an ABI Prism 7500 Sequence Detector System (Applied Biosystems, Foster City, CA, USA) using absolute quantification analysis.
T. gondii DNA copy numbers, present in PCR samples, were determined by the absolute quantification analysis, done automatically by the software, applied with the RT PCR instrument.
Vales of Ct (cycle at which the set threshold is reached) were then exported into an Excel file (Microsoft Corporation, Manchester, UK), and absolute quantification analysis was performed using the gDNA standard curve.
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A Light Cycler 480 instrument (Roche) was used for the real-time absolute quantification PCR analysis.
In addition to a comparison of volatile and non-volatile (sugars, acids, and carotenoids) compounds, and the interrelationships of these chemical components, a comparative iTRAQ (isobaric tags for relative and absolute quantification) proteome analysis was used to identify qualitative and quantitative differences in the proteome between the two hybrids at three levels of maturity.
Both absolute and relative quantification analysis were done using comparative Ct (ΔΔCt method) [ 64].
JK performed the termite cultivation, absolute quantification of pyrolysis products analysis and Py-TMAH-GC/MS analysis, and drafted the manuscript.
The copy number of specific cDNA molecules present in the samples was determined by absolute quantification method of qPCR analysis [ 45].
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