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Also, we agree that a comparison of absolute modification efficiencies across different experiments (including those from previous reports) can be problematic.
Furthermore, it is generally difficult to compare absolute modification efficiencies across different experiments (especially those previously reported) – cell conditions, transfection efficiencies, and even analysis conditions can vary greatly.
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Herein we report absolute cytosine modification levels in human brain tissues at fetal and different postnatal developmental stages.
When the absolute value of modification coefficient is 0.6, relative errors vary from 0.002 % to 0.38%%.
Relative errors decrease sharply with the increase of absolute value of modification coefficient.
When the absolute value of modification coefficient is 0.6, the relative errors vary from 2.10 % to 10.41 %.
Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer's instructions with one modification; absolute ethanol was used in place of isopropanol.
After the final wash, the nanoparticles were suspended in 20 mL absolute ethanol for amine modification.
As H3K9me2 ChIP-seq measures only the relative enrichment, we have scaled it to indicate absolute quantities of histone modification.
Severe or frequent hypoglycemia is an absolute indication for the modification of treatment regimens, including setting higher glycemic goals.
Therefore, rather than yielding the absolute concentration of a modification in a given tissue, the data show directly the extent to which the analyzed tRNA set is modified.
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