Sentence examples similar to absolute minimum final concentration from inspiring English sources

The RBITC derivative was prepared by adding APTES to RBITC previously dissolved in absolute ethanol (final concentration 1 mM) at the molar ratio of 1 1 (APTES/RBTIC).

Spores were harvested from the plates using sterile swabs and two mL of sterile distilled water and absolute ethanol (50% final concentration).

Actinomycin D (Sigma, 1 mg/ml dissolved in absolute ethanol), at a final concentration of 1 μg/ml was also added.

The cultivation was maintained for 6 days by adding absolute methanol to a final concentration of 1% every day.

Pectin was precipitated by adding absolute ethanol to a final concentration of 30% to the supernatant and incubated at 4°C overnight.

For subsequent expression array hybridization, a minimum of 200 ng/μL final concentration of labeled RNA was used.

For selection of C. albicans transformants, mycophenolic acid (MPA; Invitrogen), dissolved in absolute ethanol, was added to a final concentration of 5 μg/ml).

Doxycycline was added to a final concentration of 300 pg ml−1 (minimum effective concentration) and incubated for 12 hours in order to induce VSG221 RNAi.

Absolute methanol was added every 24 h to a final concentration of 1% to maintain induction.

When required, CoQ10 prediluted in FBS was added to the plates at a final concentration of 30 µM (CoQ10, Synthetic Minimum 98%, high-performance liquid chromatography, Sigma).

Absolute, ice-cold (-20°C) methanol was added while vortexing to a final concentration of 90%.