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Esakkimuthu et al. (2015) have reported that strong absolute loadings on BOD and TC indicate organic pollution from human activities.
This factor had high absolute loadings on Ca2+, Mg2+, TH, HCO3−, pH, SO 4 2−, EC and TDS and a moderate loading on K+.
For discussion, we focused in genes having absolute loadings values larger than 0.3 (Figure 2, S1, S2 and S3).
Variables with high absolute loadings with respect to the component under consideration were considered significant [ 45].
To reveal the genes responsible for the separation of tissues, genes with highest absolute loadings were selected.
Variables with absolute loadings >0.20 and that had a 95% confidence interval not equal to zero were considered significant.
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The terms 'strong'moderateand'weakd 'weas' appliedied to factor loadings, refer to absolute loading values of >0.75, 0.75 0.5 and 0.5 0.3, respectively (Liu et al. 2003).
The terms "strong", "moderate" and "weak" were applied to factor loadings, referring to absolute loading values of >0.70, 0.70 0.50 and 0.50 0.30, respectively.
Liu et al. (2003) classified the factor loadings as 'Weak', 'Moderate' and 'Strong' corresponding to absolute loading values of 0.3 0.5, 0.5 0.75 and >0.75, respectively.
Liu et al. (2003) classified the factor loadings as "strong", "moderate" and "weak", corresponding to absolute loading values of >0.75, 0.75 0.50 and 0.50 0.30, respectively.
The component loadings are classified as strong, moderate and weak with absolute loading values of > 0.75, 0.75 0.50 and 0.50 0.30, respectively.
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