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The Livak method (2 ΔΔCT) was used to quantify relative and absolute expressions of each gene between chitosan/carbon scaffolds and chitosan scaffolds.
To transform expression measurements from diverse platforms onto a common scale, percentile transformation was applied to absolute expressions for each array data set, and then the median of percentile transformed intensities across replicated measurements per gene was used (see Methods for details).
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Finally, differentially expressed genes (DEGs) were selected for i) FDR <0.05 and ii) absolute expression changes larger than twofold.
"The solar screens are an absolute expression of what I believe in," she beams.
"People come in their everyday dress," said Clay Sheff, the studio's founder, their looks "an absolute expression of their musical styles".
As Hernándaz turns Cojocaru in his arms, her form evanescent as a wisp of smoke, you see the absolute expression of her vulnerability and of his wonder and desire.
We analyzed the microarray data for general trends in alternative splicing in terms of absolute expression and differential expression.
The absolute expression value of each folate-related gene was normalised to the expression level of the rice actin gene.
However, accurate measurements of absolute expression levels and the reliable detection of low abundance genes are difficult to achieve.
Our emotions are the absolute expression of relatedness and of relationship; they give voice to the exchange of everything that is taking place in our pure and real experience.
According to the challenge specification, absolute expression levels are neither required nor desired.
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