Participants in both conditions reported whether a target was present or absent using one of two keys on the keyboard using their right hand.
Chiasm compression, cavernous sinus invasion and sella turcica destruction were diagnosed on the basis of radiological appearance and considered present or absent, using standard radiological criteria [12].
After capillary electrophoresis, AFLP fragments were scored into a binary data matrix as 1 (present) or 0 (absent) using Gene Mapper 4.0 software package (Applied Biosystems).
Considering that the factor analyzed was the moisture in the dentin in the adhesive technique (present or absent) using the adhesive system Single Bond Universal (3M ESPE), the control group was consisted of the conventional technique using the adhesive system Scotchbond Multi-purpose (3M espe).
Ubiquitination of Mfn2 in control cells was apparently absent using valinomycin in combination with a proteasomal inhibitor.
For further analysis probesets (ps) were called present or absent using the Bioconductor package "panp", and ps were excluded which showed very low expression across all arrays (P>0.05).
For all ITS and CR alignments, gaps were coded as present or absent using the "simple" gap coding method employed in GapCoder v1.0 [35].
Relevant genes absent from the B. pumilus gene list were confirmed as absent using B. subtilis and B. licheniformis sequences as queries for local BLAST against the B. pumilus genome.
Most of these bacteria were identified by molecular methodology and were absent using standard culture, especially in patients 1, 2, 3 and 6, which suggests that the molecular identification is clinically useful than culture techniques.
The identity of an additional band observed (using western blotting with anti-SMP30 antibody) in liver extracts of normal 7 day old rat is unclear at present, but it was absent (using western blotting with anti-HA antibody) in liver extracts of Ad-SMP30-HA injected mice suggesting the possibility that the anti-SMP30 antibody may have non-specific cross-reactivity.
Probes were also flagged (Present, Marginal, Absent) using GeneSpring default settings.
