Third, a conserved 5.6 kb NE-specific locus (Table 5; NELoc-3) was identified that harboured five genes, three of which were either absent or significantly divergent from equivalent regions in the reference genomes (Figure 1C).
The 1H/15N HSQC spectrum of the mutant protein showed a number of resonances from residues towards the C-terminus which are either absent or significantly reduced in intensities when compared to the spectrum of the wild-type protein.
We used two complementary approaches to identify and confirm sequences that were both conserved among the various NE strains but absent or significantly divergent from the nine publicly available C. perfringens strains (ATCC 13124, SM101, Str. 13, NCTC 8239, F4969, ATCC 3626, JGS1495, JGS1721, JGS1987) (Table 1).
Oscillatory rises in Ca2+ were absent or significantly reduced after incubation with the NADH-ubiquinone oxidoreductase (complex I) inhibitor, piericidin A (2 µM) (Figure 2C, n = 2) or FCCP (2 µM) (Figure 2D, n = 3), confirming the mitochondrial origin of these responses.
We defined LOH as a completely absent or significantly decreased signal intensity of one allele.
These latter activities are absent or significantly weaker in Ogg1 (16, 21, 22).
These inhibitory effects of Dex were absent or significantly reduced in AnxA1−/− mice.
Thus, an LIM homolog may be either absent or significantly smaller in macaque monkeys, compared with LIM in humans.
We found that decorin was abundantly expressed in the stroma of all examined normal tissues and absent or significantly reduced in the corresponding tumors.
Such a plant could operate on a biochemical target in the parasite that is either absent or significantly different in the host [ 55].
