The absence of techniques reducing autofluorescence (from lipofuscin) might have contributed to a high level of background autofluorescence and low specific antibody fluorescence signal [15].
Adherence to treatment protocol and absence of techniques and elements from contrasting treatments is checked.
However, due to the absence of techniques for quantitatively examining central synapses in vivo, a role for the Teneurins in organizing central synapses has not been established.
In the absence of techniques for lipids comparable to genetic knockouts or knockdowns, inhibition of lipid biosynthesis is one of the few ways in which lipid levels in cells can be manipulated.
We believe that this is mainly due to the absence of techniques that can objectively characterize the latent tissue fluorophores and quantify the changes in their abundance during the pathogenesis of a disease.
In addition, we did not directly measure intra-abdominal pressure and the absence of techniques to precisely assessing intraglomerular and peritubular microvasculature and tissue oxygenation/energetics do not allow any robust conclusions to be drawn.
For example, neuromuscular Neurexin and Neuroligin regulate synaptic development and assembly (Li et al., 2007; Banovic et al., 2010; Sun et al., 2011; Chen et al., 2012; Mosca et al., 2012; Owald et al., 2012), but remain (as with many other identified molecules) largely untested in the CNS due to the absence of techniques for doing so.
However, the absence of a technique capable of fully characterising these structures is hampering progress.
We do things less elaborately here, but in the very absence of dramatic technique there is a certain appeal to the emotions.
In the absence of standard technique, we recommend to check the nature of the particles using physical extraction in combination with the 1% Na2CO3 in order to provide a more careful analysis of ASi.
